Autoimmunity

Identification and characterization of human autoreactive T cells for autoimmune research.- T cell antigen discovery using cell-based epitope libraries.- Purification, Phenotypic and Functional analyses of Regulatory T Cells in Autoimmunity.- Culturing Murine Bone Marrow-Derived Macrophages.- Macrophage cell culture and characterization by serum human AB in health and disease.- Isolation and culture of rheumatoid arthritis synoviocytes.- Comprehensive Assessment of Neutrophil Function in Autoimmune Diseases.-  Neutrophil transmigration analyses for multiplexed drug screening and in-vivo like application testing using an organ on a chip model.- Induction, isolation and characterization of human neutrophil extracellular traps (NETs) to be used in functional assays.- Analysis of cellular metabolism using flow cytometry.- Quantifying mitochondrial metabolism and metabolic fluxes in soft agar cultures.- Assessing mitochondrial respiration of  models for the study of immune-related diseases using a Seahorse analyzer.- Investigating dysregulated X chromosome inactivation in human B cells and plasmacytoid dendritic cells by RNA FISH .- Single-Cell Spatial Transcriptomic of Sjögren’s Disease Salivary Glands.-  Establishment of Adult Stem Cell-Derived Organoid Cultures: General Principles from the Mouse Gut.- lymphoid differentiation of human hematopoietic progenitor cells from co-cultures in hypoxia.- Analysis of microRNAs Expression across Cells, Tissues and Body Fluids.- Polysome profiling: a method for global and targeted analysis of mRNA translation.- A Versatile Ribo-seq Workflow for Enhanced Analysis of Ribosome Dynamics.