An Introduction to P-type ATPase research.- Purification of Na,K-ATPase from Pig Kidney.- Preparation of Ca
-ATPase1a Enzyme from Rabbit Sarcoplasmic Reticulum.- Isolation of H
/K
-ATPase-enriched Membrane Fraction from Pig Stomachs.- Overproduction of PiB-type ATPases.- Coordinated Overexpression in Yeast of a P4-ATPase and its Associated Cdc50 Subunit: The Case of the Drs2p/Cdc50p Lipid Flippase Complex.- The Plasma Membrane Ca
-ATPase: Purification by Calmodulin Affinity Chromatography, and Reconstitution of the Purified Protein.- Expression of Na,K-ATPase and H,K-ATPase Isoforms With The Baculovirus Expression System.- Time-dependent Protein Thermostability Assay.- Colorimetric Assays of Na,K-ATPase.- ATPase Activity Measurements by an Enzyme-Coupled Spectrophotometric Assay.- Antimony-Phosphomolybdate ATPase Assay.- ATPase Activity Measurements using radiolabeled ATP.- Assaying P-type ATPases Reconstituted in Liposomes.- Coupling ratio for Ca
Transport by Calcium Oxalate Precipitation.- Calcium Uptake in Crude Tissue Preparation.- Measuring H
Pumping and Membrane Potential Formation in Sealed Membrane Vesicle Systems.- Assay of Flippase Activity in Proteoliposomes using Fluorescent Lipid Derivatives.- The use of Metal Fluoride Compounds as Phosphate Analogs for Understanding the Structural Mechanism in P-type ATPases.- Phosphorylation/Dephosphorylation Assays.- Tryptophan Fluorescence Changes Related to Ca
-ATPase Function.- Determination of the ATP Affinity of the Sarcoplasmic Reticulum Ca
-ATPase by Competitive Inhibition of [g-32P]TNP-8N3-ATP Photolabeling.
23. Ca
Binding and Transport Studied with Ca
/EGTA Buffers and
Ca
24. Assay of Copper Transfer and Binding to P
-ATPases
25. Voltage Clamp Fluorometry of P-Type ATPases
26. Electrophysiological Measurements on Solid Supported Membranes
27. Electrophysiological Characterization of Na,K-ATPases Expressed in
Xenopus laevis
Oocytes Using Two-Electrode Voltage Clamping
28. Functional Studies of Na
,K
-ATPase using Transfected Cell Cultures
29. HPLC Neurotransmitter Analysis
30. Behavior Test Relevant to a
/a
Na
/K
-ATPase Gene Modified Mouse Models
31. Zebrafish Whole-mount
in situ
Hybridization Followed by Sectioning
32. Whole-mount Immunohistochemistry for Anti-F59 in Zebrafish Embryos (1-5 days Post Fertilization (dpf)) Canan Doganli
33. Cell-based Lipid Flippase Assay Employing Fluorescent Lipid Derivatives
34. Transient Expression of P-type ATPases in Tobacco Epidermal Cells
35. Lipid Exchange by Ultracentrifugation
36. Reconstitution of Na
, K
-ATPase in Nanodiscs
37. Crystallization of P-type ATPases by the High Lipid-detergent (HiLiDe) Method
38. Two-dimensional Crystallization of the Ca
-ATPase for Electron Crystallography
39. Two-dimensional Crystallization of Gastric H+,K+-ATPase for Structural Analysis By Electron Crystallography
40. MD Simulations of P-type ATPases in a Lipid Bilayer System
41. Computational Classification of P-Type ATPases
42. Molecular Modeling of Fluorescent SERCA Biosensors
43. How to Compare, Analyze and Morph Between Crystal Structures of Different Conformations: The P-type ATPase Example
Jesper L
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