Ewing Sarcoma

Germline variation and somatic alterations in Ewing sarcoma. - Western blot analysis in Ewing sarcoma.- Tissue preservation and FFPE samples: Optimized nucleic acids isolation in Ewing sarcoma.Untargeted metabolite screening of Ewing sarcoma cells - and tissues with Liquid Chromatography – Tandem Mass Spectrometry (LC-MS/MS).- (Immuno-)histological analysis of Ewing sarcoma.- Molecular approaches to diagnosis diagnosis in Ewing sarcoma: RT-PCR.- Molecular approaches to diagnosis in Ewing sarcoma: Targeted RNA sequencing.- Ewing-specific (re)expression models.- Analysis of regulatory sequences by dual-luciferase reporter assays in Ewing sarcoma.- Proliferation assessment by Trypan blue exclusion in Ewing sarcoma.- of resazurin and ATP detection cell viability assays for drug sensitivity screening in Ewing sarcoma.- Analysis of migration and invasion in Ewing sarcoma.- Analysis of colony formation capacity in Ewing sarcoma.- Genetic and epigenetic manipulation using CRISPR techniques in Ewing sarcoma.- Post-translational regulation using degrons in Ewing sarcoma.- 3D Ewing sarcoma modelling for metabolomic studies.- Genetically engineered mouse model in Ewing sarcoma.- Tumor growth analysis of Ewing sarcoma cell lines using subcutaneous xenografts in mice.- Metastasis assessment in Ewing sarcoma using orthotopic xenografts.- Orthotopic implants in mice.- Ewing sarcoma PDX models.- Using zebrafish larvae as a xenotransplantation model to study Ewing sarcoma.- Chorioallantoic membrane assay to assess primary and metastatic tumor growth in Ewing sarcoma.- A Drosophila study model for EWS::FLI1-induced molecular pathway- A practical compass to biological digital resources and omics integration in Ewing sarcoma. - Chromatin immunoprecipitation followed by next-generation sequencing in Ewing sarcoma.- Epigenetic analysis in Ewing sarcoma.- Leveraging single-cell RNA sequencing to characterize murine Ewing sarcoma xenografts.- Spatial transcriptomics analysis of Ewing sarcoma.