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Gillespie

Type-1 Diabetes

Methods and Protocols

Medium: Buch
ISBN: 978-1-4939-3641-0
Verlag: Springer
Erscheinungstermin: 03.06.2016
Lieferfrist: bis zu 10 Tage

The aim of this volume is to present current methodologies to predict and understand the pathogenesis of Type-1 Diabetes for clinical and non-clinical researchers.   Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Practical and reliable, Type-1 Diabetes: Methods and Protocols will aid researchers in using these methods to advance their own studies.


Produkteigenschaften


  • Artikelnummer: 9781493936410
  • Medium: Buch
  • ISBN: 978-1-4939-3641-0
  • Verlag: Springer
  • Erscheinungstermin: 03.06.2016
  • Sprache(n): Englisch
  • Auflage: 1. Auflage 2016
  • Serie: Methods in Molecular Biology
  • Produktform: Gebunden, HC runder Rücken kaschiert
  • Gewicht: 5709 g
  • Seiten: 213
  • Format (B x H x T): 183 x 260 x 18 mm
  • Ausgabetyp: Kein, Unbekannt
Autoren/Hrsg.

Herausgeber

Type 1 Diabetes: Prediction And Prevention: Current Perspectives.- The HLA.- Pancreatic Beta Cell Survival and Signaling Pathways: Effects of Type 1 Diabetes-Associated Genetic Variants.- Islet Autoantibody Analysis: Radioimmunoassays.- Islet Autoantibody Analysis: Non-Radioactive Assays - Electrochemiluminescence.- Detection of C-Peptide in Urine as a Measure of Ongoing Beta Cell Function in Type 1 Diabetes.- Histology of the Type 1 Diabetes Pancreas.- Identification of Islet Antigen-Specific CD8 T Cells Using MHCI-Peptide Tetramer Reagents in the Non Obese Diabetic (NOD) Mouse Model of Type 1 Diabetes.- Tracking Immunological Responses of Islet Antigen-Specific T Cells In The Non Obese Diabetic (NOD) Mouse Model Of Type 1 Diabetes.- Adoptive Transfer of Autoimmune Diabetes using Immunodeficient Non Obese Diabetic (NOD) Mice.- Methylation Analysis in Distinct Immune Cell Subsets in Type 1 Diabetes.- FISH with Concomitant Immunofluorescence.- Laser Capture Microdissection:  Single Pancreatic Cell DNA Profiling.- The Gut Microbiome in the NOD Mouse.- Molecular Methods and Protein Synthesis for Definition of Autoantibody Epitopes.