This volume details in methods to assess memory B cell formation and function in mice and humans. Chapters guide readers through tetramer-based methods to assess antigen-specific memory B cell dynamics in humans and mice in different vaccine, next-generation deep-sequencing, single-cell techniques to assess epigenomic, VDJ landscapes, lymph node aspirates from humans, advanced imaging, murine models to determine memory B cells formation, and bioinformatic techniques and in silico modelling of memory b cell formation. Written in the highly successful series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, aims to ensure successful results in the further study of this vital field.
Produkteigenschaften
- Artikelnummer: 9781071639498
- Medium: Buch
- ISBN: 978-1-0716-3949-8
- Verlag: Springer US
- Erscheinungstermin: 18.07.2024
- Sprache(n): Englisch
- Auflage: 2024
- Serie: Methods in Molecular Biology
- Produktform: Gebunden, HC runder Rücken kaschiert
- Gewicht: 700 g
- Seiten: 252
- Format (B x H x T): 183 x 260 x 20 mm
- Ausgabetyp: Kein, Unbekannt
Themen
- Technische Wissenschaften
- Maschinenbau | Werkstoffkunde
- Technische Mechanik | Werkstoffkunde
- Materialwissenschaft: Biomaterialien, Nanomaterialien, Kohlenstoff
- Interdisziplinäres
- Wissenschaften
- Wissenschaften: Forschung und Information
- Forschungsmethodik, Wissenschaftliche Ausstattung