RNA Chaperones

A two-step purification strategy for RNA and RNA-Protein complexes for crystallization.- Analysis of tRNA aminoacylation by northern blotting and multiplex small RNA-Seq.- Characterization of RNA Structure Dynamics with trans-factors using Single-Molecule Optical Tweezers.- Specific nucleic acid chaperone activity of HIV-1 nucleocapsid protein deduced from hairpin unfolding.- A dual-color electrophoretic mobility shift assay to examine RNA binding and RNA chaperone activity simultaneously.- Use of tRNA mediated suppression to assess RNA chaperone function.- Salt-dependent modulation of the RNA chaperone activity of RNA-binding protein La.- Kinetic and thermodynamic analyses of RNA–protein interactions.- Detection of MicroRNAs released from Argonautes.- Mapping the RNA chaperone activity of the T. brucei editosome using SHAPE-chemical probing.- Using immunoprecipitation followed by dot blotting to identify oligonucleotide-protein interactions in cells.- In vivo RNA chemical footprinting analysis in Archaea.- RNA structure analysis by chemical probing with DMS and CMCT.- Prediction of intrinsically disordered regions that bind RNA with DisoRDPbind.- Delivering Molecular Beacons via an Electroporation-Based Approach Enables Live-Cell Imaging of Single RNA Transcripts and Genomic Loci.- Site-specific dual-color labeling of long RNAs.- Characterizing complex nucleic acid interactions of LINE1 ORF1p by single molecule force spectroscopy.- Isolation and analysis of bacterial ribosomes through sucrose gradient ultracentrifugation.