CRISPR-Cas Methods

Targeted base editing in rice using Target-AID.- Accelerated laboratory evolution of conjugative plasmids for CRISPR delivery in bacteria.- A SERS-signalled, CRISPR-Cas-powered bioassay for amplification-free and anti-interference detection of SARS-CoV-2 in foods and environmental samples using a single tube-in-tube vessel.- Genetic Engineering of Bacteriophage Using CRISPR-Cas12a.- CRISPR-Cas12a-powered dual-mode biosensor for ultrasensitive and cross-validating detection of pathogenic bacteria.- Intronization of coding sequences for optimization of gene expression.- Sensitive small molecule aptasensing-based on hybridization chain reaction and CRISPR-Cas12a using a portable 3D-printed visualizer.- Highly efficient genome editing in plants with the LbCas12a-RRV variant.- CRISPR-Cas13a-based bacterial detection platform: Sensing pathogen Staphylococcus aureus in food samples.- High efficiency Cas12a mediated A-to-G base editing in rice.- A smartphone-based visual biosensor for CRISPR-Cas powered SARS-CoV-2 diagnostics.- Efficient single-cell cloning of genome-edited cultured human cells.- Ultrasensitive pathogenic bacteria detection by a smartphone-read G-quadruplex-based CRISPR-Cas12a bioassay.- A ratiometric fluorescent biosensing platform for ultrasensitive detection of Salmonella typhimurium via CRISPR-Cas12a and silver nanoclusters.- CRISPR-Cas12a-based aptasensor for sensitive and selective ATP detection.- A portable, visual and dual-mode biosensor for ultrasensitive and self-validating detection of foodborne pathogenic bacteria based on CRISPR-Cas9 system.- CRISPR-Cas12-based PCV2 viral detection.- Surface-enhanced Raman scattering -based CRISPR-Cas assay on microfluidic paper analytical devices for supersensitive detection of pathogenic bacteria in foods.- CRISPR-Cas in woody perennial plants: methods, efficiency, applications, and challenges to creating commercial varieties with high ecological and economic value.- A Unified Protocol for Genome Editing in Monocot and Dicot Plants Using a Transposon-Associated TnpB System.- CRISPR-Cas12a-Based DNA Detection for Rapid Diagnosis of Wheat Blast Disease.