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Lafond / Vaillancourt

Human Embryogenesis

Methods and Protocols

Medium: Buch
ISBN: 978-1-62703-832-4
Verlag: Humana
Erscheinungstermin: 30.10.2014
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Despite its inherent controversy, the exploration of the human embryo can unlock many of the answers to our deepest biological questions. In , internationally recognized researchers contribute detailed methods to analyze various aspects of the embryogenesis process. While comprehensively covering subjects such as the molecular mechanisms of embryonic development, in vitro fertilization, cloning, and the laws and ethical considerations of working with embryos, the volume also addresses critical features of fetal and placental development as well as of uterine biology. Written in the highly successful ™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and state-of-the-art, provides a firm foundation for the successful analysis of the embryogenesis process and an easily accessible description of the limitations and advantages of the techniques proposed, certain to aid all those who wish to further unravel the mysteries of human embryogenesis.

Produkteigenschaften

  • Artikelnummer: 9781627038324
  • Medium: Buch
  • ISBN: 978-1-62703-832-4
  • Verlag: Humana
  • Erscheinungstermin: 30.10.2014
  • Sprache(n): Englisch
  • Auflage: 2009
  • Serie: Methods in Molecular Biology
  • Produktform: Kartoniert, Paperback
  • Gewicht: 679 g
  • Seiten: 312
  • Format (B x H x T): 193 x 260 x 18 mm
  • Ausgabetyp: Kein, Unbekannt

Themen

Autoren/Hrsg.

Herausgeber

Part I. Introduction: What is Human Embryogenesis?Human Embryogenesis: Overview Cathy Vaillancourt and Julie LafondPart II. Studying Embryo Implantation: from Trophoblasts to EmbryoDifferential Gene Expression in the Uterus and Blastocyst During the Reactivation of Embryo Development in a Model of Delayed Implantation. Pavine L.C. Lefèvre and Bruce D. MurphyCoculture of Decidua and Trophoblast to Study Proliferation and Invasion Marie Cohen and Paul Bischof Isolation and Culture of Term Human Cytotrophoblast Cells and In Vitro Methods for Studying Human Cytotrophoblast Cells Calcium Uptake Frédérique Le Bellego, Cathy Vaillancourt and Julie LafondEndocrinology and Cell Signaling in Human Villous Trophoblast Catherine Mounier, Benoit Barbeau, Cathy Vaillancourt and Julie LafondGestation-Induced Uterine Vascular Remodeling Pierre-André Scott, Mylène Provencher, Pascale Guérin and Jean St-Louis Part III. Steriodogenesis and Reproductive SystemPlacental and Fetal Steriodogenesis J. Thomas SandersonCurrent Methods in Investigating the Development of the Female Reproductive System Patrick J. Devine, Patricia B. Hoyer and Aileen F. KeatingA Revised and Improved Method for the Isolation of Seminiferous Tubule-Enriched Fractions that Preserves the Phosphorylated and Glycosylated Forms of Proteins Casimir D. Akpov and R.-Marc PelletierA Novel Technical Approach for the Measurement of Individual ACAT-1 and ACAT-2 Enzymatic Activity in the Testis Li Chen, Julie Lafond and R.-Marc PelletierPart IV. From Embryo to Fetal Development GeneticDissection of Caenorhabditis elegans Embryogenesis Using RNA Interference and Flow-CytometryElodie L. Boulier and Sarah JennaImmune System: Maturation of Myeloid Cells Jamila Ennaciri and Denis Girard Functional Development of Human Fetal Gastrointestinal TractEmile Levy, Edgard Delvin, Daniel Ménard and Jean-François BeaulieuRenal and Cardiac Na+-K+-ATPase and Aconitase in a Rat Model of Fetal ProgrammingRébecca Gaudet and Michèle Brochu Part V. Embryotoxicity, In Vitro Fertilization, Cloning, and EthicsAssessment of Embryotoxicity Using Mouse Embryo CultureLouise M. Winn and Emily W.Y. TungFertilization In Vitro Yinzhong Bing and Rodney J. OuelletteAnimal Cloning by Somatic Cell Nuclear Transfer Lawrence C. Smith and Jae-Gyu YooThe Human Embryo: Ethical and Legal Aspects Bartha Maria Knoppers, Sylvie Bordet and Rosario Isasi