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Botrytis Cinerea

Methods and Protocols

Medium: Buch
ISBN: 978-1-0716-5550-4
Verlag: Springer Us
Erscheinungstermin: 25.09.2026
vorbestellbar, Erscheinungstermin ca. Oktober 2026

This volume provides comprehensive protocols on Botrytis cinerea and the main methods used to study this important fungal species. Chapters detail cover classical approaches, including isolation, cultural techniques, sclerotia induction, storage, microscopy and protein localization, inoculation assays on fruits and vegetables, antifungal susceptibility testing, protoplast production and transfection, RNA interference, and identification of mycoviral infections. Additionally, the volume presents state-of-the-art molecular and omics-based approaches, including high-quality DNA and RNA extraction, molecular marker development, genome assembly, quantitative gene expression analysis, and integrative transcriptomic, proteomic, and metabolomic analyses. Written for the highly successful   series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and comprehensive,  aims to promote experimental rigor, facilitate cross-disciplinary collaboration, and stimulate innovative research directions in biology.


Produkteigenschaften


  • Artikelnummer: 9781071655504
  • Medium: Buch
  • ISBN: 978-1-0716-5550-4
  • Verlag: Springer Us
  • Erscheinungstermin: 25.09.2026
  • Sprache(n): Englisch
  • Auflage: Erscheinungsjahr 2026
  • Serie: Methods in Molecular Biology
  • Produktform: Gebunden
  • Seiten: 400
  • Format (B x H): 178 x 254 mm
  • Ausgabetyp: Kein, Unbekannt
Autoren/Hrsg.

Herausgeber

Isolation Methods.- Culture and Maintenance Methods for   sexual reproduction of  Viable Storage of  Conidia and Sclerotia.- Fluorescence and Ultrastructural Microscopy for Cell Biology of : Live Cell Imaging Techniques and Protein Localization Protocols.- Methods For Inoculating  on Leaves and Fruit.- Protocol For The Extraction Of High Quality DNA From Strain Differentiation by SSR-PCR using Capillary Sequencer and Agarose Gel Electrophoresis.- Genome Assembly and Annotation of a Plant Pathogen using the Example of  Identification of Mycoviral Infections.-  protoplasts production and inoculation.- Assessing Sensitivity to Antifungal Plant Compounds or Agrochemicals.- Extracellular Vesicle Isolation from culture and -Infected Plants .- Assays of Antifungal Activity of an Antimicrobial Peptide against  Evaluation of Biocontrol Efficacy by Competing Yeasts.- Methods For Evaluating the Efficacy of Microbial Biocontrol Agents Against  on Tomato Plant.- Procedures for the study of  proteome.