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Salmonella

Methods and Protocols

Medium: Buch
ISBN: 978-1-0716-5558-0
Verlag: Springer Us
Erscheinungstermin: 19.09.2026
vorbestellbar, Erscheinungstermin ca. September 2026

This fourth edition volume expands on the previous editions with updates on the latest methods used to study a variety of different aspects that are advancing our knowledge on   research. The chapters in this book cover topics such as culture-based isolation of  from different matrices; whole genome sequence analysis of ; standardized quantification of  biofilms and evaluation of antibiotic anti-biofilm activity; blocking ELISA protocol for  infection;  infection in chronic inflammation and gastrointestinal cancer; isolation of bacteriophage: an antibiotic alternative for the control of  infections; detection and characterization of  serotypes by PCR technologies; and determining prevalence of  in beef. Written in the highly successful  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, is a valuable reference for both expert researchers and students. The genetic manipulation, molecular methods, and molecular imaging techniques will be of interest to geneticists, cell and molecular biologists, microbiologists, environmentalists, toxicologists, public health scientists, clinicians in human and veterinary medicine, agriculture, and other researchers who want to become familiar and apply techniques that are commonly not available in research papers. This book will also be of interest to students who are interested in learning more about the latest research on .


Produkteigenschaften


  • Artikelnummer: 9781071655580
  • Medium: Buch
  • ISBN: 978-1-0716-5558-0
  • Verlag: Springer Us
  • Erscheinungstermin: 19.09.2026
  • Sprache(n): Englisch
  • Auflage: 4th Fourth 2026 Auflage
  • Serie: Methods in Molecular Biology
  • Produktform: Gebunden
  • Seiten: 531
  • Format (B x H): 178 x 254 mm
  • Ausgabetyp: Kein, Unbekannt
  • Vorauflage: 978-1-0716-0790-9
Autoren/Hrsg.

Herausgeber

Biochemical Identification of Species: Classical Tools for Accurate Diagnosis.- Culture-Based Isolation of from Different Matrices.- A Simplified Guide to Whole Genome Sequence Analysis of on the Command Line.- Detection of Using Conventional Methods: Emphasis on the Use of Modified Semi-Solid Rappaport Vassiliadis Agar (MSRV).- Standardized Quantification of Biofilms and Evaluation of Antibiotic Anti-Biofilm Activity.- Blocking ELISA Protocol for Infection.- Measure the Biofilm Formation of Using Crystal Violet Assay.- Establishment of In Vivo Mouse Models to Study -Induced Chronic Infection and Gastrointestinal Cancer.- From Sewers to Surveillance: Monitoring through Wastewater.- Generation of Random Transcriptional Fusions in the Genome of serovar Typhimurium.- Electrochemical Biosensing of  Conjugation Assay for Antimicrobial Resistance Plasmids in Motility Matters: Quantitative Analysis of Swimming and Swarming.- Isolation of Bacteriophage: Antibiotic Alternative for the Control of Infections.- Construction of Transposon Mutant Library.- Modular Cloning for Rapid Generation of Recombinant Plasmids for Analyses of Pathogenesis.- Generation and Use of Reporter Cell Lines to Analyze Integrity of -Containing Vacuoles by Automated Live-Cell Imaging and Quantification.- Comet Assay for Genotoxicity Evaluation of Outer Membrane Vesicles.- Detection and Characterization of Serotypes by Simple PCR Technologies.- Loop-Mediated Isothermal Amplification (LAMP) as a Rapid Molecular Tool for Detection in Food.- Next Day spp. Detection Method Based on Real-Time PCR for Foods.- Determining Prevalence of in Beef (Ground and Intact).- Stress Tolerance Assays for : Methods for Evaluating Heat, Acidity, and Osmotic Resistance.- Construction of Library with Unique, Diverse DNA Barcodes in Strains.- Genomic Tracing of Transmission Dynamics in Invasive Non-Typhoidal Outbreaks.