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Type 2 Diabetes

Methods and Protocols

Medium: Buch
ISBN: 978-1-61779-492-6
Verlag: Humana
Erscheinungstermin: 30.11.2011
Lieferfrist: bis zu 10 Tage

Diabetes is now reaching epidemic proportions, and the associated complications of this disease can be disabling and even life-threatening. In , leading investigators provide up-to-date explanations of commonly used laboratory protocols used in diabetes research. Covering the commonly described in vivo and in vitro model systems, the volume ultimately leads to an overall view of how cellular dysfunction and degeneration leads to susceptibility and diabetes disease progression. Written in the highly successful series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and expert notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, offers succinct, proven techniques to aid research scientists and clinicians in continuing the study of this debilitating disease.


Produkteigenschaften


  • Artikelnummer: 9781617794926
  • Medium: Buch
  • ISBN: 978-1-61779-492-6
  • Verlag: Humana
  • Erscheinungstermin: 30.11.2011
  • Sprache(n): Englisch
  • Auflage: 1. Auflage 2011
  • Serie: Methods in Molecular Biology
  • Produktform: Kartoniert, Paperback
  • Gewicht: 565 g
  • Seiten: 278
  • Format (B x H x T): 193 x 242 x 16 mm
  • Ausgabetyp: Kein, Unbekannt
Autoren/Hrsg.

Herausgeber

1) Selecting the 'Right' Mouse Model for Metabolic Syndrome and Type 2 Diabetes ResearchEdward H. Leiter2) Nutritional Models of Type 2 Diabetes MellitusBeverly Sara Muhlhausler3) The Isolation and Purification of Rodent Pancreatic Islets of LangerhansJacqueline F. O¿Dowd4) The Measurement of Insulin Secretion from Isolated Rodent Islets of LangerhansAnna L. Nolan and Jacqueline F. O¿Dowd5) The Incubation and Monitoring of Cell Viability in Primary Rat Islets of Langerhans and Pancreatic ß-Cell LinesNoel G. Morgan, Eleftheria Diakogiannaki, and Mark A. Russell6) In Vitro Culture of Isolated Islets of Langerhans: Analysis of FunctionAnna L. Nolan7) Single Cell RT-PCR Identification of Genes Expressed by Human Islet Endocrine CellsDany Muller, Peter M. Jones, and Shanta J. Persaud8) Laser Capture Microdissection of Human Pancreatic b ¿Cells and RNA Preparation for Gene Expression Profiling Lorella Marselli, Dennis C. Sgroi, Susan Bonner-Weir, and Gordon C. Weir9) In Vitro Transdifferentiation of Human Hepatoma Cells into Pancreatic-Like CellsWan-chun Li10) The Measurement of GLUT4 Translocation in 3T3-L1 AdipocytesNicky Konstantopoulos and Juan Carlos Molero-Navajas11) Pancreatic Remodeling: Beta Cell Apoptosis, Proliferation and Neogenesis and the Measurement of Beta Cell Mass and of Individual Beta Cell SizeEduard Montanya and Noèlia Téllez12) Morphology of Pancreatic Islets: A Time Course of Pre-Diabetes in Zucker Fatty RatsPetra Augstein and Eckhard Salzsieder13) Fluorescent Immunohistochemistry and in situ Hybridization Analysis of PancreasXiuli Wang, Shundi Ge, and Gay M. Crooks14) The Measurement of Insulin Secretion Using Pancreas Perfusionin the RodentEdward T. Wargent15) Hyperinsulinemic-Euglycemic Clamp to Assess Insulin Sensitivity In VivoJason K. Kim16) Gene Expression Analysis in Diabetes ResearchPeter White and Klaus H. Kaestner17) Gene Expression Mining in Type 2 Diabetes ResearchDonald R. Dunbar